Discher, som konstruerade nanopartiklar som självmonterades i olika former vid upptäckten, insåg att CD47-SIRPa-mekanismen för självigenkänning i princip
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The interaction between SIRPα and CD47 can be modified by endocytosis or cleavage of the receptor, or interaction with surfactant proteins. CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily  and partners with membrane integrins and also binds the ligands thrombospondin-1 ( TSP-1 ) and signal-regulatory protein alpha ( SIRPα ).  2020-05-19 · The CD47/signal regulatory protein α (Cd47/SIRPα)interaction provides a macrophage immune checkpoint pathway that plays a critical role in cancer immune evasion across multiple cancers. Here, we report the engineering of a humanized anti-SIRPα monoclonal antibody (1H9) for antibody target cancer therapy. 1H9 has broad activity across a wide range of SIRPα variants.
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2020-2-3 · CD47/SIRPa antibody development. To accelerate direct efﬁcacy and toxicity testing of anti-human CD47 and SIRPa antibodies, Biocytogen has generated the double humanized mice, B-SIRPa/hCD47, in which the human extracellular domains of SIRPa and CD47 replace their respective murine counterparts. 2021-3-2 · agents that block the CD47:SIRP-alpha engagement are attractive therapeutic targets as a monotherapy or in combination with additional immune-modulating agents for activating antitumor T cells in vivo the data suggest that combinatorial actions of ADAM10 and gamma-secretase on SIRPalpha cleavage promote inflammatory signaling. The CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-α interaction. The key to this kit is the high sensitivity of detection of biotinlabeled SIRP-α by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate.
The binding of cell-surface CD47 with SIRPA on 2016-09-01 · CD47–SIRPa blocking component to an Fc-domain containing antibody, we predicted enhanced tumor elimination analogous to the synergy observed through combination of CD47–SIRPa blocking agents and FcR engagement. Here, we tested these hypotheses by generating SIRPabodies in a variety of formats The CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-α interaction. The key to this kit is the high sensitivity of detection of biotinlabeled SIRP-α by streptavidin-HRP.
2011-04-05 · Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane.
STATUS Available. Jul 3, 2018 Published: July 3, 2018.
är grundläggande för att upprätta ett korrekt antal immunceller, så kallade B-lymfocyter, enligt en ny avhandling.
Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
CD47 has a single Ig-like extracellular domain and five membrane spanning regions. The interaction between SIRPα and CD47 can be modified by endocytosis or cleavage of the receptor, or interaction with surfactant proteins. CD47 or SIRPa might thus mediate unidirectional signalling in the hematopoietic or immune systems. For instance, the binding of CD47 on RBCs (in which minimal expression of SIRPa exists) to SIRPa of macrophages regulates phagocytosis by macro-phages in a unidirectional manner (see later). Ligation of SIRPa by CD47 promotes tyrosine phos- This signal is CD47, an immune checkpoint inhibitor that is ubiquitously expressed but is upregulated in various tumor types.
Moreover, the abundant expression of CD47 throughout the
Creative Biolabs provides CD47 & SIRPA engineered antibodies such as therapeutic antibodies, nanobodies, bispecific antibodies and intrabodies.
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Research so far has largely focused on improving adaptive immune functions, but recent studies have indicated that the signal-regulatory protein (SIRP)α-CD47 pathway, a phago … Inhibitory immune checkpoint blockade has been one of the most significant advances in anticancer therapy of the past decade.
In the present study, 269 advanced CRC lesions were immunohistochemically evaluated for CD47, SIRPA, CD68, and CD163 expression in tumor cells and TAIs. This CD47/SIRP alpha binding assay can be run in a 96- or 384-well low volume white plate (20 µL final).